Part:BBa_K3777026
KB2-mphR-ermC-T7(mphO)-3WJdB-PmphR-sgRNA
Basic biosensor device for erythromycin detection.
Usage and Biology
Compared to our another mphR-ermC-T7(mphO)-3WJdB(BBa_K3777021),it adds a binding site which can be regonized by dcas9 proteins and sgRNA.(Fig 1)
When Ery was absent, mphR would bind to the inducible promoter（PI）and prevent RNA polymerase from initiating transcription, thus repressing the expression of reporter gene. If tet was present, TetR would no longer able to bind to the promoter, resulting in the expression of reporter gene. But KB2 will bind to 3WJDB leading to lower fluorescent .
Reference：Alam Khalid K,Tawiah Kwaku D,Lichte Matthew F,Porciani David,Burke Donald H. A Fluorescent Split Aptamer for Visualizing RNA-RNA Assembly In Vivo.[J]. ACS synthetic biology,2017,6(9)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 260
Illegal NheI site found at 283
Illegal NheI site found at 1599
Illegal NheI site found at 2015 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1493
Illegal XhoI site found at 121
Illegal XhoI site found at 1144
Illegal XhoI site found at 2139 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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